Devils

• Taz (Jim Cummings) – He is the central character of the series and appears in every episode. The plots of most of the episodes are based upon the relationships various characters have with Taz. Taz is cast as teenager in most episodes and is less ferocious than his original incarnation. He works as a bellhop at the Hotel Taz-mania.
• Hugh Tazmanian Devil (Maurice LaMarche) – Taz's easy-going father. In many cases meant to be a parody of Bing Crosby and Bob Hope. Hugh likes orange juice and playing golf. Crosby was a famous pitch-man for orange juice, while Hope would perform stand-up comedy in golf attire. Hope and Crosby appeared together as a popular duo in the well-known Road film series of the 1940s and 1950s. Hugh's voice typically sounds like Crosby.
• Jean Tazmanian Devil (Miriam Flynn) – Taz's dedicated, doting mother.
• Molly Tazmanian Devil (Kellie Martin) – Taz's teenage sister. Despite being more refined than her brother, she often shares his vicious nature, though in a more sibling-rivalry sense.
• Jake Tazmanian Devil (Debi Derryberry) – Taz's little brother, who like a typical little brother, often looking up to and idolizing Taz.
• Drew Tazmanian Devil (Maurice LaMarche) – Taz's uncle, who acts like Bob Hope and sometimes joins his brother Hugh in playing golf. He and Hugh would occasionally travel together in episodes spoofing the Road movies.
• Dog the Turtle (Maurice LaMarche) – Taz's pet turtle, who acts like a dog.

Hotel Tazmania staff

• Bushwhacker Bob (Jim Cummings) – Taz's loud, grouchy and incompetent boss. Owner of the Hotel Tazmania, he is largely self-centered and thinks very highly of himself, despite rarely doing any actual work and preferring to leave any and all tasks to his staff. His demeanor is a send up of Basil Fawlty, just as all the hotel segments were direct spoofs of the British sitcom Fawlty Towers.
• Mum (Rosalyn Landor) – Bushwhacker Bob's mother, who is much calmer and wiser than her son. Despite her son owning the Hotel Tazmania, Mum is the true backbone of the establishment. She is often quick to criticize her son, much to Bob's chargin, despite her accusations typically being true.
• Constance Koala (Rosalyn Landor ) – An enormous but gentle spirited koala who is the maid at the Hotel Taz-mania. She is quite fond of singing and dancing, despite her dancing often causing unintentional destruction.
• Mr. Thickley (Dan Castellaneta) – A multitalented and upbeat wallaby who works at the Hotel Tazmania, although his exact job title is unspecified. He enjoys doing favors for Taz but his incompetence usually results in mayhem. He also hosts Taz-manian Theater.

Outback characters

• Digeri Dingo (Rob Paulsen) – A self-serving dingo who pretends to be Taz's friend so he can avoid dangerous situations and discomfort at Taz's expense. A scavenger, treasure hunter and chronic collector, he often takes advantage of Taz's strength and rabid nature to hunt rare treasures.
• Wendal T. Wolf (Jim Cummings) – A neurotic Tasmanian wolf who is desperate for any type of friendship. When not being hunted by Taz, he usually drives him crazy in his efforts to befriend him. His personality closely resembles that of Woody Allen.
• Francis X. Bushlad (Rob Paulsen) – A white skinned, red haired aboriginal boy who unsuccessfully hunts Taz as a rite of manhood. Despite their tribal society, he and his entire tribe behave and speak like highly intelligent, aristocratic businessmen. His name recalls silent movie star Francis X. Bushman

Minor characters

• Bull Gator and Axl (John Astin, Rob Paulsen) – A pair of alligators who unsuccessfully try to trap Taz for the enjoyment of zoo-going children all over the world. Bull is the leader of the duo, always acting in a confident and cheery attitude even while reprimanding Axl. Axl is Bull's hunter in training, constantly naive though often subject to Bull's "corrections." (Typically in the form of a mallet smashing.) They vaguely resemble Laurel and Hardy.
• Buddy Boar (Jim Cummings) – Behaves somewhat like Didgeri Dingo toward Taz although he is somewhat less selfish. He fancies himself a yuppie and spends much of the time talking on his cell phone. He was established early in the show as "Taz's best friend," although this was seemingly ill-received, reflected in several fourth-wall breaking moments by the show's characters. As such his appearances were uncommon.
• Daniel and Timothy Platypus (Maurice LaMarche, Rob Paulsen) – Twin brothers (Timothy wears glasses) whose love for do-it-yourself projects usually end up causing trouble for Taz. They closely resemble Daffy Duck, in appearance and manner of speech.
• The Keewee – Although somewhat resembling a kiwi, this silent bird can run as fast as the Road Runner, often being chased down by Taz in search of his lunch in the same nature of Wile E. Coyote.
• The Bushrats (head Bushrat: Phil Proctor) – A group of rats in tribal costume. They speak in an odd mix of real and nonsense languages that are appended by humorous subtitles.
• Willie Wombat (Maurice LaMarche) – Originally cast in a Bugs Bunny-like role against Taz, Willie resents this typecasting and greatly admires Taz and his career. His determination to remain pacifistic and polite usually reverts to frustration and rage by the end of his episodes as fate conspires to lock him in a role as Taz's antagonist.