A typical episode of Toonsylvania starts with a cartoon featuring the adventures of Dr. Vic Frankenstein (voiced by David Warner), his assistant Igor (voiced by Wayne Knight) who always sets out to prove that he's the real genius instead of Dr. Vic, and their dim-witted Frankenstein Monster known as Phil (voiced by Brad Garrett). Before the second cartoon, there is an animated vignette where Igor is on the couch with Phil and tries to fix the TV remote, but in every episode there's a new problem with it (a running gag akin to the couch gags seen on The Simpsons).

After that, there is a cartoon series called "Night of the Living Fred", about a family of zombies. This segment was created by Pulitzer Prize-winning cartoonist Mike Peters.

Sometimes, a parody of a B-List horror movie would air instead of a "Night of the Living Fred" cartoon.

After that is a short segment called "Igor's Science Minute", where Igor gives a science lesson (be it a musical piece or a spoken piece) that always ends in disaster.

The final segment is "Melissa Screetch's Morbid Morals", where Phil the Frankenstein monster does something bad and Igor punishes him by reading a tale involving a bratty girl named Melissa Screetch (voiced by Nancy Cartwright of Simpsons fame) who doesn't heed the warnings of adults (usually given by her mother) and gets punished one way or the other for it.

In season 2, Bill Kopp and Jeff DeGrandis left the show and were replaced by former Animaniacs writer Paul Rugg. The series' format changed into more of a sitcom style, with Igor, Dr. Vic and Phil interacting with a variety of new characters, including a snooping next-door neighbor Seth Tuber (voiced by Jonathan Harris), who was based on Norman Bates from the movie Psycho. He interacted with his "immobile" mother by putting his hand over his mouth and talking into it. There was also a typical Transylvanian angry mob that was in fact a cheerful group of Beatles-esque hipsters. Most of these new characters were voiced by Paul Rugg, who also improvised many of their lines.

The only other backup segment to re-materialize in season 2 was Melissa Screetch in a new segment called "The Melissa Screetch Show". Whenever Melissa was disappointed with a friend or a family member, she'd go home and cover herself under her bedsheets where she pretended to host a show. She then had her transgressor on as a guest star and often did away with them in an ironic manner.

This season also had a return to the B-movie parodies in the first season, featuring just one cartoon about America being over taken by aliens who want to force America to use the Metric System. The lead alien was voiced by Billy West effecting a French Accent, making the segment a parody of American xenophobia.

Season 1

• Darla Doily-Demon Doll/The Importance of Being Urnie/The Boogeyman'll Get You Melissa
• Blind Date of Frankenstein/Football and Other Body Parts/Stop Making Ugly Faces
• Love Potion Number Nein/Attack of the Iguana People/Teeth for Two
• Baby Human/Earth Vs. Everything/Little Screetchin Riding Hood
• Built for Speed/A Kiss Before Dying Unless of Course You're Already Dead/Go Stand in the Corner Young Lady
• Spawn of Santa/Dead Hard/Don't Swallow the Seeds Silly
• Doom With a View/Dead Dog Day Afternoon/Here There Be Monsters
• Love Hurts/One for Mall and Mall for One/Plain as the Nose on Your Face
• Phil Feel Smart/Voodoo Vacation/Melissa, Don't Spoil Your Appetite
• WereGranny/The Lobster of Party Beach/The Screetchy Little Mermaid
• A Family Plot/A Zombie is Born/Melissa and The Three Bears

Season 2

• Phil's Brain/Jurassic Putt/Bites and Stings/You Keep Bouncing Like That You're G
• The Inferior Decorator/Bang!/Parasites/Melissa Screetch, Earth Ambassador
• Something Weenie This Way Comes/Ideadical Cousins/Melisserella
• Igor's Replacement / The Deadman Bunch
• My Fair Monster / The Nosey Face / Shelf Of Brains
• The Doomed Odyssey/Attack of the Fifty Footed Woman/Becki with an I
• The Longest Day/Take Us to Your Liter/Escape from Wet Nurse Island
• No Title/In Or Out/Melissa Makes A Wish/Madame Olga's Lament

The music for the series was written by Michael Tavera, Keith Baxter, Christopher Neal Nelson, John Paul Given, Christopher Klatman and Thom Sharp. The main title song was written by Steve Bernstein and Julie Bernstein with lyrics by Paul Rugg.

• David Warner - Dr. Vic Frankenstein
• Wayne Knight - Igor
• Brad Garrett - Phil
• Nancy Cartwright - Melissa Screetch ("Melissa Sreetch's Morbid Morals" segments)
• Matt Frewer - Dedgar Deadman (1998, ""Night of the Living Fred" segments)
• Jess Harnell - Dedgar Deadman (1998-1999, "Night of the Living Fred" segments)
• Jonathan Harris - Seth Tuber
• Tom Kenny - Ace Deuce
• Valery Pappas - Stiffany Deadman ("Night of the Living Fred" segments), Melissa Screetch's Mother ("Melissa Sreetch's Morbid Morals" segments)
• Paul Rugg - Seth Tuber's Mom
• Kath Soucie - Ashley Deadman ("Night of the Living Fred" segments)
• Billy West - Fred Deadman ("Night of the Living Fred" segments), Newark

Additional Voices

• Charlie Adler -
• Jocelyn Blue - Mama Bear
• Corey Burton - Death
• Cam Clarke -
• Jim Cummings - Army General ("B-Movie" segments)
• Sandy Fox - Darla Doily, Baby Bear
• Bill Kopp -

• Bill Kopp - Executive Producer, Writer, Creator ("B-Movie"), Voice Drector
• Jeff DeGrandis - Producer, Director
• Steven Spielberg - Executive Producer
• Keith Baxter - Writer
• Lee Mendelson - Creative Consultant ("Night of the Living Fred")
• Vinny Montello - Writer
• Steve Ochs - Writer
• Martin Olson - Writer
• Chris Otsuki - Creator ("Melissa Screetch"), Writer
• Mike Peters - Creator ("Night of the Living Fred"), Writer
• Karl Toerge - Writer

• In August 1999, a VHS video of Toonsylvania was released, which contained the episodes "Darla Doiley, Demon Doll," "Voodoo Vacation," "Baby Human," "Dead Dog Day Afternoon," "Igor's Science Minute (Clone or Be Cloned, The Brain, Earthquake Boogie, and Gravity)," "Melissa Screetch's Morbid Morals (The Boogeyman, Stop Making Ugly Faces, Here There be Monsters, and Melissa Screetch: Earth Ambassador)," "Phil's Brain," "Football...and other Body Parts," "Bang!," and "WereGranny". The video is currently out of print and Dreamworks has yet to announce any plans to put the series on DVD.

A Toonsylvania video game was released by UbiSoft for the Game Boy Color in 2000.

Toonsylvania action figures and playsets were developed by Pangea Corporation and released by Toy Island and Burger King distributed toys based on Toonsylvania in their kids' meals for a short period of time.

• Chile
  • Canal 13